The Saudi Journal of Forensic Medicine and Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 2  |  Issue : 2  |  Page : 28--31

Assessment of blood pregabalin stability at different postmortem durations


Rawan A Almowalad1, Hanan Emara1, Hatem Ahmed1, Samah F Ibrahim2 
1 Department of Forensic Chemistry, Naif Arab University for Security Sciences, Riyadh, Kingdom of Saudi Arabia
2 Department of Clinical Sciences, Faculty of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia; Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Giza, Egypt

Correspondence Address:
Samah F Ibrahim
Department of Clinical Sciences, Faculty of Medicine, Princess Nourah Bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia, Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Cairo University, Giza

Background: Pregabalin (PRG) has been abused due to its availability and its cheap price. This study is aimed to test the postmortem stability of PRG in blood specimens. Materials and Methods: Ninety-six male rats were divided into four groups, which were given oral doses of PRG: 4 g and 2.5 g/kg for 1 day; 50 mg and 20 mg/kg/day for 21 consecutive days. Antemortem PRG stability was assessed at 4 and 6 h after the last ingested dose, while postmortem stability was assessed at 24 and 48 h after death using gas chromatographymass spectrometry. Results: Our study could detect that -80°C was a storage temperature that could reserve PRG stability in antemortem and postmortem blood samples. The increase in oral PRG dosage was accompanied with a significant increase in blood PRG concentration. Postmortem PRG blood concentrations were decreased in comparison to antemortem concentrations. However, this decrease did not highlight a statistically significant difference in PRG stability (P < 0.05) at the tested storage condition. Conclusion: PRG analysis could be performed in a peripheral blood specimen within 2 days of sampling.


How to cite this article:
Almowalad RA, Emara H, Ahmed H, Ibrahim SF. Assessment of blood pregabalin stability at different postmortem durations.Saudi J Forensic Med Sci 2019;2:28-31


How to cite this URL:
Almowalad RA, Emara H, Ahmed H, Ibrahim SF. Assessment of blood pregabalin stability at different postmortem durations. Saudi J Forensic Med Sci [serial online] 2019 [cited 2021 Sep 27 ];2:28-31
Available from: https://www.sjfms.org/article.asp?issn=2589-8329;year=2019;volume=2;issue=2;spage=28;epage=31;aulast=Almowalad;type=0